rpoC [2017-05-26 16:29:16]

RNA polymerase beta subunit

Locus: BSU01080

Isoelectric point: 8.86

Molecular weight: 133.92 kDa

Protein length: 1199 aa

Gene length: 3597 bp

Function: transcription

Product: RNA polymerase beta subunit

Essential: yes

E.C.: 2.7.7.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → RNA polymerase
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 125,562 → 129,161

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)

Protein family

• RNA polymerase beta' chain family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-335 and Arg-803 PubMed

Structure

• 3IYD (RNA polymerase from E. coli, 50% identity) PubMed

Localization

• colocalizes with the nucleoid PubMed
• membrane associated PubMed

Expression and Regulation

Operons

• Genes: rpoB-rpoC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the leader mRNA is processed upstream of rpoB by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

Reviews

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Original publications

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